Method Revealing Bacterial Cell-Wall Architecture by Time-Dependent Isotope Labeling and Quantitative Liquid Chromatography/Mass Spectrometry
نویسندگان
چکیده
منابع مشابه
Identification and quantitative studies of protein immobilization sites by stable isotope labeling and mass spectrometry.
A method was developed for characterizing immobilization sites on a protein based on stable isotope labeling and MALDI-TOF mass spectrometry. The model for this work was human serum albumin (HSA) immobilized onto silica by the Schiff base method. The immobilized HSA was digested by various proteolytic enzymes in the presence of normal water, while soluble HSA was digested in (18)O-enriched wate...
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The detection of doping with recombinant peptide and protein hormones (e.g. human growth hormone -hGH, erythropoietin Epo) is one of the most challenging analytical problems in doping control. Rapid metabolic degradation paired with a low medical dosing strategy reduces the time window for a successful direct detection by immunological methods in the case of hGH to just a few hours after the la...
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Quantitative analysis of protein expression is an important tool for the examination of complex biological systems. Albeit its importance, quantitative proteomics is still a challenging task because of the high dynamic range of protein amounts in the cell and the variation in the physical properties of proteins. Stable isotope labeling by amino acids in cell culture (SILAC) has been successfull...
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A new liquid chromatography/mass spectrometry (LC/MS) method is described for relative quantification of phosphoproteins to simultaneously compare the phosphorylation status of proteins under two different conditions. Quantification was achieved by beta-elimination of phosphate from phospho-Ser/Thr followed by Micheal addition of ethanethiol and/or ethane-d(5)-thiol selectively at the vinyl moi...
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BACKGROUND Measurement of porphobilinogen (PBG) is useful in the diagnosis of the acute neurologic porphyrias. Currently used colorimetric assays lack analytical and clinical sensitivity and specificity. METHODS We developed a liquid chromatography-electrospray tandem mass spectrometry (LC-MS/MS) method for the measurement of PBG in 1 mL of urine, using 5-(aminoethyl)-4-(carboxymethyl) 1H-2,4...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2009
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac802587r